Do you know about stocking capacity and such? If not, you may have too many fish in your tank and that is causing an overload in waste so even more bacteria need to be established. Also, Bio Spira has an expiry date and needs to be kept in the fridge I think so you may have a bad one. Also, when you use Bio Spira, at first you'll experience a spike in ammonia. Then the ammonia goes down and then you'll see a spike in nitrites, then that goes down. The spikes only last about 2 days, or so I have been told. My personal opinion is just to do a lot of water changes. It may seem a pain at first but once you get into a habit, it is easy. Good luck!