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(pulled from one of the many places I posted instructions, each written for the particular situation, the initial tank flushing may not be necessary)
What you're doing is fish-in cycling, not a recommended practice, a tank should be cycled before adding fish. Cycling sets up a portion of the nitrogen cycle to remove ammonia generated by the fish as they breathe. Bacteria are grown to do this, these bacteria colonies are called the biofilter.
Here's a link to explain what cycling is:
Here's what happens when you cycle using ammonia, the cleanest way:
But you have fish, so the tank will be cycling with fish in it, which is possible and can be done safely if done correctly. If not, the fish will either die or be severely damaged and probably die a few months later.
Your water conditioner has to be either Prime or Amquel Plus, Amquel Plus is better for cycling
because it detoxes nitrite and nitrate in addition to ammonia. Either of these will detoxify ammonia, Stress Coat and most other conditioners remove chlorine, but not ammonia and are useless during fish in cycling. That particular bacteria additive is fine, but not necessary, Prime or Amquel are what will keep the fish from being damaged as the biofilter grows. Safe Start is designed to keep ammonia below 2 ppm to keep most fish from being killed immediately.
Nitrite is less of a concern because salt in the tank from food, etc detoxifies quite a bit of it.
First thing to do is change all the water by doing 5-6 50% changes. Do the first refill in 2 steps, waiting 15 mins after filling to 75% to fill completely, this will reduce the possibility of shock. The rest can be filled completely each time. Use Prime or Amquel for these refills and any in the future.
Ammonia will rise continuously, then nitrite, then both will drop to zero. At that point the biofilter has grown completely and cycling is complete.
Test ammonia daily, when it first gets over 1 ppm, change enough water to get it down to .25ppm or so. This may have to be done daily and may require more than one change; if large changes are done daily, shock won't occur, so 2-3 in a row are no problem.
3-4 days after ammonia begins to rise, start testing nitrite, too. Nitrite will spike to very high levels if unchecked, but again, do large pwc's down to .25ppm whenever nitrite first exceeds 1 ppm.
About 3 weeks from addition of fish, cycling will complete if water changes are done as described,
the tank is kept at about 78F and very strong aeration is provided.
Again, cycling completion is when ammonia and nitrite both test zero.
Do not use any other additives or chemicals of any sort in the tank beside Prime or Amquel Plus. Nothing.
The pwc's are important, high ammonia slows bacterial growth, especially nitrite eaters.
1 ppm is an arbitrary figure to make dosing easy and reduce the amount of water needed for each change and it will speed cycling by a few days.